Olympus FVMPE-RS Multiphoton Microscope
The Olympus FVMPE-RS multiphoton imaging system is purpose-built for deep imaging in biological tissue, aimed at revealing both detail and dynamics. It is an upright microscope with gantry frame, optimized for intravital imaging with a large space for live animals and experimental equipment. Innovative features for efficient delivery and detection of photons in scattering media enable high signal-to-noise ratio acquisition. The system is equipped with a dual wavelength laser with a tunable range from 680 nm to 1300 nm and a fixed wavelength line at 1045 nm (InSight X3 Dual-OL by Spectra-Physics). Hybrid scan unit consists of a high-speed acquisition resonant scanner and a high-definition galvanometer scanner. High-sensitivity GaAsP detectors and Olympus dedicated multiphoton objectives allow for deep tissue imaging under in vivo and cleared tissue conditions.
Capability
Two-photon fluorescence imaging
2D, 3D, and 4D image collection and processing, 3D/4D animation
Lasers
Tunable 680-1300nm
Fixed 1045nm
Samples
Fixed tissue slices and cleared organ samples (up to 4mm), Organoid / spheroid cultures, embryos, small animals (up to 1.5mm)
Filter Cubes for Sample Check with Wide-field Fluorescence
DAPI (350/50X, BS400, 460/50M)
EGFP/FITC/CY2 (470/40X, BS495, 525/50M)
CY3/TRITC (545/25X, BS565, 605/70M)
CY5 (620/60X, BS660, 700/75M)
Available Fluorescence NDD Filter Cubes for Detectors
FV30-FVG - VIOLET (BA410-460) /485/ GREEN (BA495-540)
FV30-FCY – CYAN (BA460-500) /505/ YELLOW (BA520-560)
FV30-FGR – GREEN (BA495-540) /570/ RED (BA575-645)
FV30-FRCY5 – RED (BA575-645) /650/ FAR RED (BA660-750)
Non-Descanned Detectors (NDD)
2 multi-alkali PMT
2 Gallium-Arsenide-Phosphide (GaAsP) PMT
Olympus FVMPE-RS Manual
Coming Soon!
Objectives
| Objective | MAG | NA | WD (mm) | IR | Medium | |
|---|---|---|---|---|---|---|
| UPLFLN4X | U Plan Fluorite 4X Objective | 4x | 0.13 | 17 | N/A | dry |
| UMPLFLN10XW | LWD PLAN FLUOR 10X WTR DIPPING OBJ | 10x | 0.3 | 3.5 | yes | water |
|
UMPLFLN20XW |
LWD PLAN FLUOR 20X WTR DIPPING OBJ | 20x | 0.5 | 3.5 | yes | water |
| LCPLN20XIR | LWD/CC PLAN 20X OBJ | 20x | 0.45 | 8.3 | near IR | dry |
|
LUMPLFLN40XW |
LWD PLAN FLUOR 40X WTR DIPPING OBJ | 40x | 0.8 | 3.3 | yes | water |
|
LUMPLFLN60XW |
LWD PLAN FLUOR 60X WTR DIPPING OBJ | 60x | 1.0 | 2.0 | yes | water |
| FV30-AC25W | truResolution 25x water immersion objective with a motorized correction collar | 25x | 1.05 | 2.0 | yes | water |
Available Primary Emission Beam Splitters for Detectors
| DICHROIC CUBE | DETECTORS CONFIGURATION |
|---|---|
| FV30-SDM-M - for 2 detectors | all emission to RNDD 3/4 (GaAsP) |
| FV30-SDM570 - for 4 detectors |
>570nm RNDD 1/2 (alkali) |
| Empty/out of light path - for 2 detectors | all emission to RNDD 1/2 (alkali) |
Contact Us
Małgorzata (Margo) Bieńkowska-Haba, PhD
Research Specialist, Microscopy
Email: Malgorzata.Bienkowskahaba@lsuhs.edu
Phone: (318) 675-8537
Hungwen (Kevin) Lin, PhD
Scientific Adviser, Microscopy
Associate Professor, Neurology
Email: hungwen.lin@lsuhs.edu
Phone: (318) 675-8810