OLYMPUS FVMPE-RS MULTIPHOTON MICROSCOPE
| Microscopy in Research Core
The Olympus FVMPE-RS multiphoton imaging system is purpose-built for deep imaging in biological tissue, aimed at revealing both detail and dynamics. It is an upright microscope with gantry frame, optimized for intravital imaging with a large space for live animals and experimental equipment. Innovative features for efficient delivery and detection of photons in scattering media enable high signal-to-noise ratio acquisition. The system is equipped with a dual wavelength laser with a tunable range from 680 nm to 1300 nm and a fixed wavelength line at 1045 nm (InSight X3 Dual-OL by Spectra-Physics). Hybrid scan unit consists of a high-speed acquisition resonant scanner and a high-definition galvanometer scanner. High-sensitivity GaAsP detectors and Olympus dedicated multiphoton objectives allow for deep tissue imaging under in vivo and cleared tissue conditions.
Capability
Fixed samples and live animals
Two-photon fluorescence imaging
2D, 3D, and 4D image collection and processing, 3D/4D animation
Two-photon fluorescence imaging
2D, 3D, and 4D image collection and processing, 3D/4D animation
Lasers
Tunable 680-1300nm
Fixed 1045nm
Samples
Fixed tissue slices and cleared organ samples (up to 4mm), Organoid / spheroid cultures, embryos, small animals (up to 1.5mm)
Filter Cubes for Sample Check with Wide-field Fluorescence
DAPI (350/50X, BS400, 460/50M)
EGFP/FITC/CY2 (470/40X, BS495, 525/50M)
CY3/TRITC (545/25X, BS565, 605/70M)
CY5 (620/60X, BS660, 700/75M)
Available Fluorescence NDD Filter Cubes for Detectors
FV30-FVG - VIOLET (BA410-460) /485/ GREEN (BA495-540)
FV30-FCY – CYAN (BA460-500) /505/ YELLOW (BA520-560)
FV30-FGR – GREEN (BA495-540) /570/ RED (BA575-645)
FV30-FRCY5 – RED (BA575-645) /650/ FAR RED (BA660-750)
Non-Descanned Detectors (NDD)
2 multi-alkali PMT
2 Gallium-Arsenide-Phosphide (GaAsP) PMT
Olympus FVMPE-RS Manual
Coming Soon!
Objectives
| Objective | MAG | NA | WD (mm) | IR | Medium | |
|---|---|---|---|---|---|---|
| UPLFLN4X | U Plan Fluorite 4X Objective | 4x | 0.13 | 17 | N/A | dry |
| UMPLFLN10XW | LWD PLAN FLUOR 10X WTR DIPPING OBJ | 10x | 0.3 | 3.5 | yes | water |
|
UMPLFLN20XW |
LWD PLAN FLUOR 20X WTR DIPPING OBJ | 20x | 0.5 | 3.5 | yes | water |
| LCPLN20XIR | LWD/CC PLAN 20X OBJ | 20x | 0.45 | 8.3 | near IR | dry |
|
LUMPLFLN40XW |
LWD PLAN FLUOR 40X WTR DIPPING OBJ | 40x | 0.8 | 3.3 | yes | water |
|
LUMPLFLN60XW |
LWD PLAN FLUOR 60X WTR DIPPING OBJ | 60x | 1.0 | 2.0 | yes | water |
| FV30-AC25W | truResolution 25x water immersion objective with a motorized correction collar | 25x | 1.05 | 2.0 | yes | water |
Available Primary Emission Beam Splitters for Detectors
| DICHROIC CUBE | DETECTORS CONFIGURATION |
|---|---|
| FV30-SDM-M - for 2 detectors | all emission to RNDD 3/4 (GaAsP) |
| FV30-SDM570 - for 4 detectors |
>570nm RNDD 1/2 (alkali) |
| Empty/out of light path - for 2 detectors | all emission to RNDD 1/2 (alkali) |
How do you reach us?
If you need assistance planning your experiment or just need an overview of available options, staff are happy to discuss your project goals. Please feel free to contact us for a consultation to ensure optimal experimental design and setup.
Małgorzata (Margo) Bieńkowska-Haba, PhD
Research Specialist, Microscopy
Malgorzata.Bienkowskahaba@lsuhs.edu
(318) 675-8537
Kelly Tatchell, PhD
Scientific Adviser, Microscopy
Professor and Chairman of Biochemistry & Molecular Biology
Kelly.Tatchell@lsuhs.edu
(318) 675-5163