OLYMPUS FVMPE-RS MULTIPHOTON MICROSCOPE
| Microscopy in Research Core

Purple lines

   

The Olympus FVMPE-RS multiphoton imaging system is purpose-built for deep imaging in biological tissue, aimed at revealing both detail and dynamics. It is an upright microscope with gantry frame, optimized for intravital imaging with a large space for live animals and experimental equipment. Innovative features for efficient delivery and detection of photons in scattering media enable high signal-to-noise ratio acquisition. The system is equipped with a dual wavelength laser with a tunable range from 680 nm to 1300 nm and a fixed wavelength line at 1045 nm (InSight X3 Dual-OL by Spectra-Physics). Hybrid scan unit consists of a high-speed acquisition resonant scanner and a high-definition galvanometer scanner. High-sensitivity GaAsP detectors and Olympus dedicated multiphoton objectives allow for deep tissue imaging under in vivo and cleared tissue conditions. 

 

Reservation Calendar

DESCRIPTION   

Samples 

Fixed tissue slices and cleared organ samples (up to 4mm), Organoid / spheroid cultures, embryos, small animals (up to 1.5mm)

Capabilities

Fixed samples and live animals
Two-photon fluorescence imaging
2D, 3D, and 4D image collection and processing, 3D/4D animation

Lasers

Tunable 680-1300nm
Fixed 1045nm

LED filter cubes

DAPI, FITC, TRITC, CY5

Available Fluorescence NDD Filter Cubes for Detectors

  • FV30-FVG - VIOLET (BA410-460) /485/ GREEN (BA495-540)

  • FV30-FCY – CYAN (BA460-500) /505/ YELLOW (BA520-560)

  • FV30-FGR – GREEN (BA495-540) /570/ RED (BA575-645)

  • FV30-FRCY5 – RED (BA575-645) /650/ FAR RED (BA660-750)

Non-Descanned Detectors (NDD)

2 multi-alkali PMT
2 Gallium-Arsenide-Phosphide (GaAsP) PMT 

Objectives

  • 4x/0.13 U Plan Fluorite

  • 10x/0.30 LWD PLAN FLUOR 10X WTR DIPPING OBJ

  • 20x/0.5 LWD PLAN FLUOR 20X WTR DIPPING OBJ

  • 20x/0.45 LWD/CC PLAN 20X OBJ

  • 40x/0.8 LWD PLAN FLUOR 40X WTR DIPPING OBJ

  • 60x/1.0 LWD PLAN FLUOR 40X WTR DIPPING OBJ

  • 25x/1.05 truResolution 25x water immersion objective with a motorized correction collar (installed)

How do you reach us?

If you need assistance planning your experiment or just need an overview of available options, staff are happy to discuss your project goals. Please feel free to contact us for a consultation to ensure optimal experimental design and setup.

Małgorzata (Margo) Bieńkowska-Haba, PhD
Research Specialist, Microscopy
Malgorzata.Bienkowskahaba@lsuhs.edu
(318) 675-8537