ZEISS AXIO OBSERVER & APOTOME
| Microscopy in Research Core

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The system is built with a Zeiss Axio Observer Z1 inverted fluorescent microscope, fully automated with component recognition to minimize errors. System components include X-Cite XYLIS LED lamp excitation, a Zeiss AxioCamMRm CCD camera with 12-bit dynamic range and extended sensitivity in the near infrared, fully automated xyz stage, a complement of objectives from 10x through 100x, 5 installed filter sets for DAPI, FITC, Rhodamine, Cy5 and Cy7 . The Apotome attachment is designed for precise optical sectioning. The Apotome projects a grid onto the image plane, which allows 3 raw images taken. A software algorithm then removes out-of-focus signals based on the 3 raw images. 

Reservation Calendar  Video-Start up system  Video-Change Slide holder

   

Capability

Bright field (Phase contrast, DIC)
Multichannel fluorescence
Z-stack, Mark & Find, Mosaic (image stitching), and Time lapse
Inside 4D, Colocalization

   

Sample Size

Apotome optical sectioning: Best for samples no more than 20 microns thick.

Objectives

Obj Objective Medium
10x EC Plan-Neofluar 10x/0.3 DIC I Air
20x Plan-Apochromat 20x/0.8 DIC II Air
40x LD Plan-Neofluar 40x/0.6 Ph2 DIC II Air
40x Plan-Apochromat 40x/1.3 DIC (UV) VIR-IR Oil
63x Plan-Apochromat 63x/1.4 DIC III Oil
100x Plan-Apochromat 100x/1.4 Oil

Filters

Cube Turret Position Ex Filter Dichroic (FT) Em Filter Quote Name
Brightfield 1        
34 2 390/22 420 460/50 BFP
38 HE 3 470/40 495 525/50 EGFP
31 4 565/30 585 620/60 Cy 3.5
50 5 640/30 660 690/50 Cy 5
115 6 710/87 760 814/91 Cy 7

   

How do you reach us?

If you need assistance planning your experiment or just need an overview of available options, staff are happy to discuss your project goals. Please feel free to contact us for a consultation to ensure optimal experimental design and setup.

Małgorzata (Margo) Bieńkowska-Haba, PhD
Research Specialist, Microscopy
Malgorzata.Bienkowskahaba@lsuhs.edu
(318) 675-8537

Kelly Tatchell, PhD
Scientific Adviser, Microscopy
Professor and Chairman of Biochemistry & Molecular Biology 

Kelly.Tatchell@lsuhs.edu
(318) 675-5163