ZEISS AXIO OBSERVER & APOTOME
| Microscopy in Research Core
The system is built with a Zeiss Axio Observer Z1 inverted fluorescent microscope, fully automated with component recognition to minimize errors. System components include X-Cite XYLIS LED lamp excitation, a Zeiss AxioCamMRm CCD camera with 12-bit dynamic range and extended sensitivity in the near infrared, fully automated xyz stage, a complement of objectives from 10x through 100x, 5 installed filter sets for DAPI, FITC, Rhodamine, Cy5 and Cy7 . The Apotome attachment is designed for precise optical sectioning. The Apotome projects a grid onto the image plane, which allows 3 raw images taken. A software algorithm then removes out-of-focus signals based on the 3 raw images.
Capability
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Sample Size
Apotome optical sectioning: Best for samples no more than 20 microns thick.
Objectives
| Obj | Objective | Medium |
|---|---|---|
| 10x | EC Plan-Neofluar 10x/0.3 DIC I | Air |
| 20x | Plan-Apochromat 20x/0.8 DIC II | Air |
| 40x | LD Plan-Neofluar 40x/0.6 Ph2 DIC II | Air |
| 40x | Plan-Apochromat 40x/1.3 DIC (UV) VIR-IR | Oil |
| 63x | Plan-Apochromat 63x/1.4 DIC III | Oil |
| 100x | Plan-Apochromat 100x/1.4 | Oil |
Filters
| Cube | Turret Position | Ex Filter | Dichroic (FT) | Em Filter | Quote Name |
|---|---|---|---|---|---|
| Brightfield | 1 | n/a | n/a | n/a | n/a |
| 34 | 2 | 390/22 | 420 | 460/50 | BFP |
| 38 HE | 3 | 470/40 | 495 | 525/50 | EGFP |
| 31 | 4 | 565/30 | 585 | 620/60 | Cy 3.5 |
| 50 | 5 | 640/30 | 660 | 690/50 | Cy 5 |
| 115 | 6 | 710/87 | 760 | 814/91 | Cy 7 |
How do you reach us?
If you need assistance planning your experiment or just need an overview of available options, staff are happy to discuss your project goals. Please feel free to contact us for a consultation to ensure optimal experimental design and setup.
Małgorzata (Margo) Bieńkowska-Haba, PhD
Research Specialist, Microscopy
Malgorzata.Bienkowskahaba@lsuhs.edu
(318) 675-8537