OLYMPUS CSU W1 SPINNING DISK CONFOCAL SYSTEM | Microscopy in Research Core

Capabilities

Fixed and live sample imaging
Bright-field, DIC, and fluorescence imaging
Multipoint acquisition, Multichannel acquisition, Large image scan (image stitching), Z-stack, and 2D/3D deconvolution.

Strength

The Hamamatsu Fusion cameras achieve very low and homogeneous background when images are acquired. This system is specifically designed for fast imaging to capture dynamic biological events in real-time. It is also exceptional for large image scan due to its fast speed. Mean while, the system is loaded with high NA objectives to provide excellent resolution while imaging at fast speed.

ZDC: The Z drift correction function combats focal drift and maintain long term time-lapse focus stability.

Lasers

405nm, 445nm, 488nm, 514nm, 561nm, 640nm

Samples

Confocal optical sectioning is best for samples less than 200 microns thick

Software

CellSens Dimension software in a Windows 10 operating system.

Objectives

Obj	Objective	Medium	Spin Disk Use
4x	UPlan FL N 4x/0.13NA	Dry	25, 50 μm
10x	UPlan FL N 10x/0.30NA	Dry	25, 50 μm
20x	UPlan Apo 20x/0.80NA	Dry	25, 50 μm
40x	UPlan Apo 40x/1.40NA	Oil	50 μm
60x	UPlan Apo 60x/1.50NA	Oil	50 μm
100x	UPlan Apo 100x/1.50NA	Oil	50 μm