NIKON A1R CONFOCAL & SUPER RESOLUTION SYSTEM | Research Core Microscopy

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This is a high-speed A1R confocal microscope combined with a SIM super resolution system. The Nikon Eclipse Ti-E inverted microscope is built with fully automated objectives, stage, and filter turrets. The microscope is housed in an enclosure with a stage top chamber for CO2, and humidity control.

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DESCRIPTION

Capabilities

Fixed and live sample imaging
Bright-field, phase contrast, and fluorescence imaging
FRET (Fluorescence Resonance Energy Transfer)
FRAP (Fluorescence Recovery After Photobleaching)
Multipoint acquisition, Multichannel acquisition, Large image scan (image stitching), Z-stack, and 2D/3D deconvolution.

Strength of SIM

The lateral resolution of the super resolution system can reach to 85-115nm, and the axial resolution can reach to 270nm. This doubles the resolution of a conventional confocal microscope.

PFS: Perfect Focus System with a 3 mm range to combat focal drift and maintain long term time-lapse focus stability.

Lasers

  • 405nm, 488nm, 561nm, 640nm
  • AOTF (Acousto-Optical Tunable Filter) installed

Detectors

  • Transmitted light detector
  • Two GasApe detectors (for lasers 488 and 561)
  • Two standard detectors (for lasers 405 and 640)

Objectives

  • 10x/0.45 Plan Apo
  • 20x/0.75 DIC Plan Apo VC
  • 40x/1.30 DIC Plan Fluor
  • 60x/1.40 DIC Apo
  • 100x/1.40 DIC H Plan Apo
  • 100x/1.49 SR Apo TIRF

Software

NIS-Elements (5.41)

How do you reach us?

If you need assistance planning your experiment or just need an overview of available options, staff are happy to discuss your project goals. Please feel free to contact us for a consultation to ensure optimal experimental design and setup.

Małgorzata (Margo) Bieńkowska-Haba, PhD
Research Specialist, Microscopy
Malgorzata.Bienkowskahaba@lsuhs.edu
(318) 675-8537