Leica Stellaris 8 TauSTED Confocal System

The Leica Stellaris 8 STED is a high-end confocal microscope with an extended spectral range combined with STED (Stimulated Emission Depletion) super resolution system. The system is built on an inverted DMi8 CS Premium microscope base, with fully automated objectives, stage and filter turrets. The microscope is equipped with a 405 nm laser and a tunable (440 nm-790 nm) pulsed white-light laser for excitation and five Power HyD detectors. STED Super-Resolution microscopy system includes three pulsed STED lasers (589 nm, 660 nm, and 775 nm), and is able to achieve resolution of less than 50 nm in the lateral direction and 130 nm in the axial direction. 

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DESCRIPTION

Samples

Fixed samples up to 100 microns thick for confocal imaging and up to 30 microns thick for STED imaging. 

LED filter cubes

405, GFP, Rhod LP, Y5

Lasers

  • 405 nm
  • White Light Laser, WLL: 440 nm to 790 nm
  • STED depletion lines: 589 nm, 660 nm, 775 nm

Detectors

  • Transmitted light detector
  • Two HyD S detectors
  • Two HyD X detectors
  • One HyD R detector

Objectives

  • 5x/0.15 HC PL Fluotar
  • 10x/0.40 HC PL APO CS2
  • 20x/0.75 HC PL APO CS2
  • 20x/0.75 Imm Corr HC PL APO CS2
  • 63x/1.4 Oil HC PL APO CS2
  • 100x/1.4 Oil HC PL APO CS2 STED

Software

LAS X STELLARIS Control Software, LAS X 3D Visualisation, LAS X Dye Finder, TauSTED Xtend for STELLARIS, LAS X MicroLab, LAS X Lightning Expert, LAS X Lightning STED Expert

 

How do you reach us?

If you need assistance planning your experiment or just need an overview of available options, staff are happy to discuss your project goals. Please feel free to contact us for a consultation to ensure optimal experimental design and setup.

Małgorzata (Margo) Bieńkowska-Haba, PhD
Research Specialist, Microscopy
Malgorzata.Bienkowskahaba@lsuhs.edu
(318) 675-8537