LEICA TCS SP5 CONFOCAL | Microscopy in Research Core

Purple lines
Leica SP5 Confocal Microscope

This is a very flexible confocal system build with a Leica DMI 6000 CS inverted, fully automated microscope with motorized stage, condenser, objective and filter turrets. The system is installed with 9 laser lines and is capable to customize emission bandwidth.
 

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DESCRIPTION

Capability

Fixed sample imaging
Bright field, phase contrast, and fluorescence imaging
FRAP (Fluorescence Recovery After Photobleaching )
FRET (Fluorescence Resonance Energy Transfer)
Mark & Find, Tile Imaging (image stitching), Z-stack, 3D Visualization, Co-localization

Sample

Optical sectioning best for samples no more than 100 microns thick

Lasers

  • blue diode (405nm)
  • Multi-line argon (458,476,488,496,514nm)
  • Green HeNe (543nm)
  • Orange HeNe (594nm)
  • Red HeNe (633nm)

Detectors

Transmitted light detector
Spectral beam splitter with freely adjustable bandwidth and 5 PMT (photomultiplier detectors)

Objectives

  • HC PL APO 10x0.40NA
  • HC PL APO 20x/0.7NA Imm Corr
  • HCX PL APO CS 40x/0.75U-V-I 0.17
  • HCX PL APO CS 40x1.24-0.75NA
  • HCX PL APO CS 63x/1.4-0.6NA
  • HCX PL APO CS 100x/1.46

Software

LAS AF SP5 acquisition software
Three LAS AF SP5 offline software packages with 3D analysis, multicolor analysis, microlabe and live data mode

   

How do you reach us?

If you need assistance planning your experiment or just need an overview of available options, staff are happy to discuss your project goals. Please feel free to contact us for a consultation to ensure optimal experimental design and setup.

Małgorzata (Margo) Bieńkowska-Haba, PhD
Research Specialist, Microscopy
Malgorzata.Bienkowskahaba@lsuhs.edu
(318) 675-8537