Leica TCS SP5 Confocal

Capability

Fixed sample imaging
Bright field, phase contrast, and fluorescence imaging
FRAP (Fluorescence Recovery After Photobleaching )
FRET (Fluorescence Resonance Energy Transfer)
Mark & Find, Tile Imaging (image stitching), Z-stack, 3D Visualization, Co-localization

Sample

Optical sectioning best for samples no more than 100 microns thick

Lasers

blue diode (405nm)
Multi-line argon (458,476,488,496,514nm)
Green HeNe (543nm)
Orange HeNe (594nm)
Red HeNe (633nm)

Objectives 

Obj	Objective	Medium
2.5x	PL FLUOTAR 2.5x/0.07NA	Air	Available
10x	HC PL APO 10x0.40NA	Air	Installed
20x	HC PL APO 20x/0.7NA Imm Corr	Air	Installed
40x	HCX PL APO CS 40x/0.75U-V-I 0.17	Air	Installed
40x	HCX PL APO CS 40x1.24-0.75NA	Oil	Installed
63x	HCX PL APO CS 63x/1.4-0.6NA	Oil	Installed
63x	HCX PL APO CS 63x/1.3NA glycerol corrected	Glycerol	Available
100x	HCX PL APO CS 100x/1.46	Oil	Installed
100x	HCX PL APO CS 100x/1.40-0.70NA	Oil	Available

Detectors

Transmitted light detector
Spectral beam splitter with freely adjustable bandwidth and 5 PMT (photomultiplier detectors)

Software

LAS AF SP5 acquisition software
Three LAS AF SP5 offline software packages with 3D analysis, multicolor analysis, microlabe and live data mode