Mass Spectrometry

MISSION

The mission of the Mass Spectrometry (MS) Core in the LSU Health-Shreveport Research Core Facility is to provide efficient and cost effective proteomics and metabolomics based services for research investigators.

The facility is located in room F6-12, in the Biomedical Research Institute. For protein analysis, our principal approach is bottom-up proteomics, which is a common method to identify protein and characterize their amino acid sequences and post-translational modifications by proteolytic digest prior to analysis by mass spectrometry. In addition to our developed standard operating procedures offered for many of our MS services, we also strive to continue to bring, state-of-the-art mass spectrometry based analyses through input and request from our users.

USER GUIDELINES

The Mass Spectrometry Core was established to provide service to the complete biomedical community at LSU Health-Shreveport.  Although samples will be run by facility staff, we expect users to familiarize themselves with sample preparation, data acquisition and interpretation and be an active part of sample analysis.

PUBLICATION ACKNOWLEDGEMENTS

For all publications that include data generated in the Mass Spectrometry Core, we kindly request that you acknowledge this support:

We thank the Mass Spectrometry Core at LSU Health Shreveport, supported by the LSU Health Shreveport Foundation, the Center for Cardiovascular Diseases and Sciences,  the Feist-Weiller Cancer Center, and Centers of Biomedical Research Excellence (P20GM121307).

RECENT PUBLICATIONS 

Proteomics

1. Germany CE, Reker AN, Hinton DJ, Oliveros A, Shen X, Andres-Beck LG, Wininger KM, Trutschl M, Cvek U, Choi DS, Nam HW. Pharmacoproteomics Profile in Response to Acamprosate Treatment of an Alcoholism Animal Model. Proteomics. 18(7):e1700417, 2019

2. Nam HW, Grant CA, Jorgensen AN, Holtz-Heppelmann CJ, Trutschl M, Cvek U, Neurogranin regulates alcohol sensitivity through AKT pathway in the nucleus accumbens. Proteomics. 20(1): e1900266, 2020              

3. Jorgensen AN, Rashdan NA, Rao KNS, Delgadillo LF, Kolluru GK, Krzywanski DM, Pattillo CB, Kevil CG, Nam HW. Neurogranin expression regulates mitochondrial function and redox balance in endothelial cells. Redox Biol. 70:103085 ,2024

Metabolomics

1. Sreedhar A, Cassell T, Smith P, Lu D, Nam HW, Lane AN, Zhao Y. UCP2 Overexpression Redirects Glucose into Anabolic Metabolic Pathways. Proteomics, 19(4): e1800353, 2019

2. Nahar L, Grant CA, Hewett C, Cortes D, Reker AN, Kang S, Choi DS, Nam HW, Regulation of Pv-Specific Interneurons in the Medial Prefrontal Cortex and Reward-Seeking Behaviors, J Neurochem, 156(2):212-224, 2020

3. Abulseoud OA, Ross TJ, Nam HW, Caarelli EC, Tennekopon M, Schleyer B, Castillo J, Fedota J, Gu H, Yang Y, Stein E, Short-term nicotine deprivation alters dorsal anterior cingulate glutamate concentration and concomitant cingulate-cortical functional connectivity, Neuropsychopharmacology, 45(11):1920-1930, 2020

4. Kilgore PJM, Trutschl M, Cvek U, Nam HW, hSOM: Visualizing Self-Organizing Maps to Accommodate Categorical Data. Proceedings of the International Conference Information Visualisation (IV), IEEE Computer Society, 6:611-617, 2020 

5.  Nahar L, Kaufman SE, Davis PG, Saunders SL, Disbrow EA, Patterson J, Nam HW, Blood Glutamine Synthetase Signaling in Alcohol Use Disorder and Racial Disparity, Transl Psychiatry, 12: 71, 2022

6.  Trutschl M, Kilgore PJM, Trans B, Nam HW, Clifford E, Akande A, Cvek U, hSOM: VennSOM: A SOM-Assisted Visualization of Binary Data. Proceedings of the International Conference Information Visualisation (IV), pid138, 2022

7. Jamison CN, Cuevas-Ocampo AK, Flowers AB, Nam HW, Dayton RD, McKinney MP, Mitchell HG, Fitzgerald JS, Steib ME, Toups CR, McMartin KE. Histopathological evidence that diethylene glycol produces kidney and nervous system damage in rats. Neurotoxicology. 91:200-210. 2022

Lipidomics

1. Chandra M, Escalante-Alcaldeb D, Bhuiyan MS, Orr AW, Kevil C, Morrisd AJ, Nam HW, Dominic P, McCarthy KJ, Miriyala S, Panchatcharama M. Cardiac-specific inactivation of LPP3 in mice leads to myocardial dysfunction and heart failure. Redox Biology, 14:261-271, 2018

2.  Chesnokova LS, Mosher BS, Fulkerson HL, Nam HW, Shakya AK, Yurochko AD. Distinct early role of PTEN regulation during HCMV infection of monocytes. Proc Natl Acad Sci U S A. 19;121(12):e231229012, 2024